PCR Enzyme
  • AP141-01.JPG
AP141-01.JPG

TransStart® Taq DNA Polymerase


产品介绍

TransStart® Taq DNA Polymerase是利用“TransStart热启动技术研发的新型热启动酶,是利用两种DNA结合蛋白分别与引物和模板高效结合,一种结合蛋白和引物结合,阻止了低温下引物形成二聚体;另一种结合蛋白和模板结合,阻止了低温下DNA聚合酶与DNA模板结合。随着变性步骤的进行,两种蛋白失活,释放的引物与模板参与扩增反应,增强PCR扩增效率和扩增特异性。扩增产物3'端带“A”碱基,可直接克隆于pEASY®-T系列载体中。

• 保真性是EasyTaq® DNA Polymerase 18倍。

• 特异性优于抗体封闭和化学封闭热启动DNA 聚合酶。
• 室温配制反应,减少非特异扩增和引物二聚体。
• 不使用Taq抗体,减少了潜在的来源于哺乳动物DNA污染的风险。
• 不同于化学修饰的Taq,无需加热步骤,避免损伤DNA模板和降低DNA Polymerase活性。

• 基因组DNA 片段的扩增 (≤15 kb)。

产品组成

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References

Li S , Zhou L , Yao Y , et al. A platform for the development of novel biosensors by configuring allosteric transcription factor recognition with amplified
luminescent proximity homogeneous assays[J]. Chemical Communications, 2016, 53.

Yu C Y , Yin B C , Ye B C . A universal real-time PCR assay for rapid quantification of microRNAs via the enhancement of base-stacking hybridization[J].
Chemical Communications, 2013, 49(74):8247.

Bai C , Liu X , Fisher M C , et al. Global and endemic Asian lineages of the emerging pathogenic fungus Batrachochytrium dendrobatidis widely infect
amphibians in China[J]. Diversity&Distributions, 2012, 18(3):307-318.

Lin X , Li N , Kudo H , et al. Genes Sufficient for Synthesizing Peptidoglycan are Retained in Gymnosperm Genomes, and MurE from Larix gmelinii can Rescue
the Albino Phenotype of Arabidopsis MurE Mutation[J]. Plant and Cell Physiology, 2017, 58(3):587-597.

Song Y , Zhao G , Zhang X , et al. The crosstalk between Target of Rapamycin (TOR) and Jasmonic Acid (JA) signaling existing in Arabidopsis and cotton[J].
Scientific Reports, 2017, 7:45830.

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