qPCR,qRT-PCR SuperMix
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AQ301-01.JPG

TransScript® II Green Two-Step qRT-PCR SuperMix


产品介绍

TransScript® II Green Two-Step qRT-PCR SuperMix是具有高效合成能力和高扩增效率的两步法荧光定量PCR剂盒。在同一反应体系中,用TransScript® II All-in-One SuperMix for qPCRgDNA Remover42℃-55℃件下,高效地将RNA 合成第一链cDNA,同时去除基因组DNA;另配有TransScript® II All-in-One No-RT Control SuperMix for qPCR,用于配制无反转录酶的对照,判断qPCR模板是否来自cDNAqPCR使用PerfectStartTM Tip Green qPCR SuperMix扩增。

产品组成

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第一链cDNA合成和gDNA去除

References

Chen X , Fan Z , Mcgee W , et al. TDP-43 regulates cancer-associated microRNAs[J]. Protein&Cell, 2018, 9(10):848-866.

Zhao L , Ding Q , Zeng J , et al. An Improved CTAB–Ammonium Acetate Method for Total RNA Isolation from Cotton[J]. Phytochem Anal, 2012, 23(6):647-50.

Li S , Jia X , Wen J . Improved 2-methyl-1-propanol production in an engineeredBacillus subtilisby constructing inducible pathways[J]. Biotechnology Letters, 2012, 34(12):2253-2258.

Jiang Y , Wang L . Role of histone deacetylase 3 in ankylosing spondylitis via negative feedback loop with microRNA-130a and enhancement of tumor necrosis factor-1α expression in peripheral blood mononuclear cells[J]. Molecular Medicine Reports, 2015, 13(1):35-40.

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