RT-PCR
  • AT341-01.JPG
AT341-01.JPG

TransScript® All-in-One First-Strand cDNA Synthesis SuperMix for qPCR(One-Step gDNA Removal)


产品介绍

本产品含有反转录反应所需的全部试剂(TransScript® RT, RNase Inhibitor, Anchored Oligo(dT)18 Primer, RandomPrimer(N9), dNTPs, Buffer),浓度为。反应时,只需加入gDNA Remover、模板RNA和水即可高效地合成第一cDNA,同时去除RNA模板中残留的基因组DNA。另配有TransScript® All-in-One No-RT Control SuperMix for qPCR,用于配制无反转录酶的对照,判断qPCR模板是否来自cDNA。该产品操作简便,降低操作过程中的污染机率。cDNA只适用于qPCR,不适用于常规PCR

产品组成

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使用前请将各组分点甩离心

References

Han X , Wang R , Zhou Y , et al. Mapping the Mouse Cell Atlas by Microwell-Seq[J]. Cell, 2018, 172(5):1091-1107.e17.

Tian C , Zhang X , He J , et al. An organ boundary-enriched gene regulatory network uncovers regulatory hierarchies underlying axillary meristem initiation[J]. Molecular Systems Biology, 2014, 10(10):755-755.

Wang J , Zhang Y , Hou J , et al. Ube2s regulates Sox2 stability and mouse ES cell maintenance[J]. Cell death and differentiation, 2015, 23(2).

Zhang L , Zhao X , Zhang G , et al. Light-inducible genetic engineering and control of non-homologous end-joining in industrial eukaryotic microorganisms: LML 3.0 and OFN 1.0[J]. Scientific Reports, 2016, 6:20761.

Li P , Shi M L , Shen W L , et al. Coordinated regulation of IFITM1, 2 and 3 genes by an IFN-responsive enhancer through long-range chromatin interactions[J]. Biochimica et Biophysica Acta (BBA) - Gene Regulatory Mechanisms, 2017, 1860(8):885-893.

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